Description:
- Research tools for identifying various cellular properties
Abstract
USC researchers have generated monoclonal antibodies that recognize and bind to a single epitope on specific antigens for a variety of cells and subcellular properties. Their specificity will ensure more accurate results than their polyclonal counterparts.
Market Application
Visualization of key cell types and subcellular properties are essential for scientific research. A common method utilized for this is immunofluorescent staining with antibodies. However, many antibodies are polyclonal, which are lacking selectiveness of a single epitope on the antigen. This non-specificity can lead to off-target binding and compromise experimental results.
Available antibodies
| ID # |
Antibody |
IgG |
Tissue Type |
Intended Use |
Ref |
| 1101-1 |
Immunoprecipitated Epstein Barr Virus (EBV) early antigen
|
IgG1 |
Fixed cell preparations
|
Early antigen in EBV-infected cells and tissues
|
1 |
| BM-2 |
Human granulocytes-cytoplasmic antigen
|
IgG1 |
Formalin-fixed, paraffin embedded tissue
|
Granulocytes in normal tissue or inflammatory processes
|
N/A |
| BM-3 |
Human granulocytes (98%) and monocytes (80%) - nuclear antigen
|
IgG1 |
Frozen sections; cell suspensions
|
Human myeloid cells; early marker of myeloid differentiation
|
2,3 |
| BM-4 |
Human granulocytes (83%) and monocytes (20%) - nuclear antigen
|
IgG1 |
Frozen sections; cell suspensions |
Human myeloid cells; early marker of myeloid differentiation
|
2,3 |
| BM-5 |
Human granulocytes, monocytes, and myeloid precursor cells - nuclear and cytoplasmic antigen
|
IgG1 |
Frozen sections; cell suspensions |
Human myeloid cells
|
2,3 |
| BM-6 |
Human granulocytes (87%) and monocytes (71%) -nuclear antigen
|
IgG1 |
Frozen sections; cell suspensions |
Human myeloid cells
|
2,3 |
| AE-1 |
Non-glycosylated protein component of mitochondria
|
IgG1 |
Frozen tissue/cell preparations; formalin fixed, paraffin embedded tissues
|
Mitochondria in cell/tissue; marker in subcellular fractions
|
N/A |
| AE-2 |
Double stranded DNA
|
IgG3 |
Frozen tissue/cell preparations |
Nuclear stain in cell/tissue |
N/A |
| AE-3 |
Human nucleolar antigen |
IgG1 |
Frozen tissue/cell preparations; formalin fixed, paraffin embedded tissues |
Histone H1 in animal/plant cell nuclei |
N/A |
| AE-4 |
Histone H1 |
IgG2a |
Frozen sections/cell preparations; formalin fixed, paraffin embedded tissue sections
|
Histone H1 in animal/plant cell nuclei
|
4-6 |
| AE-5 |
Human nuclear membrane |
N/A |
Frozen tissue/cell preparations; formalin fixed, paraffin embedded tissues
|
Nuclear membrane in cell/tissue; marker in subcellular fractions
|
N/A |
| AE-6 |
Human golgi zone |
IgG1 |
Frozen tissue/cell preparations; formalin fixed, paraffin embedded tissues
|
Golgi zone in cell/tissue; marker in subcellular fractions
|
N/A |
| AE-7 |
Human actin bundles
|
IgM |
Frozen tissue/cell preparations; formalin fixed, paraffin embedded tissues
|
Actin bundles in cell/tissue; marker in subcellular fractions
|
2 |
| AE-8 |
Human cytoplasmic microtubules
|
IgM |
Frozen tissue/cell preparations; formalin fixed, paraffin embedded tissues |
Cytoplasmic microtubules in cell/tissue; marker in subcellular fractions
|
2 |
| EP-1 |
Human liver canaliculi
|
IgG2b |
Fixed cell preparations/frozen tissues
|
Liver canaliculi in normal liver and liver carcinomas
|
N/A |
| EP-2 |
Human hepatocytes
|
IgG2b |
Fixed cell preparations/frozen tissues |
Liver hepatocytes in frozen human liver sections and liver carcinomas
|
N/A |
| EP-3 |
Human macrophages in the thymus and other lymphoid tissues -cytoplasmic antigen
|
IgM |
Formalin-fixed, paraffin embedded tissue
|
Macrophages |
N/A |
| EP-4 |
Human HLA-B27 - cell surface antigen
|
N/A |
Fixed cell suspensions/cytospins
|
HLA type human lymphocytes
|
N/A |
| EP-5 |
Matrix fibronectin |
IgG1 |
Formalin-fixed, paraffin embedded tissue
|
Matrix fibronectin in normal and malignant tissues
|
7 |
Publications
1. “Immunobiochemical characterization with monoclonal antibodies of Epsten-Barr virus associated early antigens
in chemically induced cells,” J Virol, 1984
2. “Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and
immunobiochemical methods using monoclonal antibodies,” Progress in Nonhistone Protein Research, 1985
3. “Expression of maturation-specific nuclear antigens in differentiating human myeloid leukemia cells,” Cancer
Res, 1985
4. “A novel method for the detection of necrotic lesions in human cancers,” Cancer Res, 1988
5. “A comparative autoradiographic study demonstrating differential intratumor localization of monoclonal
antibodies to cell surface (Lym-1) and intracellular (TNT-1) antigens,” J Nuc Med, 1990
6. “Pharmacokinetic characteristics and biodistribution of radioiodinated chimeric TNT-1, -2, and -3 monoclonal
antibodies after chemical modification with biotin,” Cancer Biother Radiopharm, 2002
7. “Identification of monoclonal antibody, TV-1, directed against the basement membrane of tumor vessels, and its
use to enhance the delivery of macromolecules to tumors after conjugation with interleukin 2,” Cancer Res, 1995