Description:
- Replace whole chromosomes and assemble chromosomes into new chromosome architectures
- Combine of genetic material from different individuals into chimeric chromosomes
- Multiplex deletion of specific genes in a single transformation
Abstract
Our R&D team developed a method to capture segments of natural chromosomes and to then reassemble them into new chromosomes inside living eukaryotic cells. This method can be used to replace whole chromosomes and allows multiplex deletion of specific genes in a single transformation. Synthetic chromosomes can be programmed to assemble into new chromosome architectures. A further application is the combination of genetic material from different individuals or species into chimeric chromosomes.
Benefit
- Efficiently achieve several deletions, insertions or chromosome assemblies in one transformation
- Replace of whole chromosomes in single step
- Combine genetic material from different individuals or species into chimeric chromosomes
Market Application
Genome editing and DNA synthesis technologies could transform the development and production of vaccines, therapeutics, and diagnostics, but the genome editing and engineering market currently faces several limitations. First, most chromosome synthesis efforts start from oligonucleotides. Oligos are often less than 60 base pairs long, so many steps of in vitro and in vivo processing are necessary to reach chromosome-size molecules. Furthermore, the presence of repetitive regions and GC content variation makes subsequent assembly steps challenging. These limitations call for more efficient synthetic strategies.
Publications
Other
Stage of Development
- Ready for commercialization
- Available for non-exclusive and exclusive license
Intellectual Property Status
US patent pending