Description:
- Gene editing toolbox for integrating larger genome sequences
Abstract
USC inventors have developed recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, thereby allowing increasing integration efficiency, particularly of large DNA fragments, into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.
Benefit
- Increase integration efficiency of large DNA fragments into the genome
- Be able to use lower linear DNA concentrations without sacrificing efficiency
- Quickly screen through various protein configurations due to cell culture systems
Market Application
Genetic knockouts through Cas-9 DNA cleavage and emergency DNA repair systems are relatively easy to produce. Genetic knock-ins/fusions are much more challenging because larger DNA inserts integrate with low efficiency. The integration rate is dependent on the inserted DNA concentration and higher concentrations of linear DNA is toxic to cells. Therefore, a more wide-ranging and efficient toolbox for the integration of donor DNA with sequences ranging from 300bp to 3,500 bp in length would present solutions, more rapid generation of useful transgenic animals, and make theranostics cheaper, faster and more accessible to clinicians and their patients.
Publications
Submitted, pending publication
Other
- Available for exclusive license